Helios Gene Gun Kit from Bio-Rad

Product Specs
ItemHelios Gene Gun Kit
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number1652411
Brand/TrademarkBio-Rad Laboratories, Life Science Group
Quantity1EA
MethodParticle-based gene delivery
ApplicationsRapid and direct transfection into a range of targets in vivo
Cell TypeCell cultures, In Vivo targets

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Axons of cortical basket cells originating from dendrites develop higher local complexity than axons emerging from basket cell somataDevelopmentNovember 15, 2023Steffen Gonda, Christian Riedel, Andreas Reiner, Ina Köhler, Petra Wahlethe more 2D environment as roller cultures flatten over time. At DIV 8, cultures were transfected (Helios Gene Gun, Bio-Rad) with plasmids encoding CMV-driven hChR2(H134R)-eYFP (RRID: Addgene plasmid #20940). Optogenetic

(2) Contrastsing synaptic roles of MDGA1 and MDGA2bioRxivOctober 26, 2023Michael A. Bemben, Matthew Sandoval, Aliza A. Le, Sehoon Won, Vivian N. Chau, Julie C. Lauterborn, Salvatore Incontro, Kathy H. Li, Alma L. Burlingame, Katherine W. Roche, et al.tubing was dried with N2 gas, and the DNA-coated gold particles were delivered to the slices with a Helios Gene Gun (BioRad). Equal amounts of plasmid DNA (when necessary) were coated to gold particles for excitatory

(3) Homosynaptic plasticity induction causes heterosynaptic changes at the unstimulated neighbors in an induction pattern and location-specific mannerFrontiers in Cellular NeuroscienceSeptember 27, 2023Ali Özgür Argunsah, Inbal Israelya fresh one every 2–3 days. After 4–5 days of culturing slices, we sparsely transfected slices by a Helios gene gun (Bio-Rad) using gold beads (10 mg, 1.6 μm diameter, Bio-Rad) that were coated with 100 μg Afp-GFP plasmid

(4) ATLAS: A rationally designed anterograde transsynaptic tracerbioRxivSeptember 17, 2023Jacqueline F. Rivera, Weiguang Weng, Haoyang Huang, Sadhna Rao, Bruce E. Herring, Don B. ArnoldBefore use, the gold particles were brought to room temperature and delivered to slice cultures via a Helios Gene Gun (BioRad). Construct expression was confirmed by YFP epifluorescence. Target preparation, mRNA display,

(5) Stress-mediated dysregulation of the Rap1 small GTPase impairs hippocampal structure and functioniScienceSeptember 15, 2023Kathryn J. Bjornson, Amanda M. Vanderplow, Yezi Yang, Danielle R. Anderson, Bailey A. Kermath, Michael E. Cahillfor 1 hour. Brains were then coronally sectioned (150 μm thick) at room temperature (Vibratome). A Helios Gene Gun was used to biolistically deliver tungsten particles coated with DiI (micro-carriers) to coronal sections

(6) Optogenetic stimulation shapes dendritic trees of infragranular cortical pyramidal cellsFront Cell NeurosciAugust 1, 2023Steffen Gonda, Ina Köhler, André Haase, Katrin Czubay, Andrea Räk, Christian Riedel, Petra Wahlepreparation. Parasagittal slices were cut at 350 μm thickness. Cultures were biolistically transfected (Helios Gene Gun, Bio-Rad, Munich, Germany) at 180 psi helium pressure (Gonda et al., 2020) with plasmids encoding CMV-driven

(7) Dispensable role of Rac1 and Rac3 after cochlear hair cell specificationJournal of Molecular MedicineJuly 1, 2023Takashi Nakamura, Hirofumi Sakaguchi, Hiroaki Mohri, Yuzuru Ninoyu, Akihiro Goto, Taro Yamaguchi, Yoshitaka Hishikawa, Michiyuki Matsuda, Naoaki Saito, Takehiko UeyamaOC explant cultures were prepared from WT P4 rats as previously described [28]. For transfection, a Helios Gene Gun (Bio-Rad Laboratories, Hercules, CA, USA) and Helios Gene Gun Diffusion Screen (165–2475) were used,

(8) Cloning and Functional Verification of Endogenous U6 Promoters for the Establishment of Efficient CRISPR/Cas9-Based Genome Editing in Castor (Ricinus communis)Genes (Basel)June 23, 2023Masatake Kanai, Kazumi Hikino, Shoji Manothe plasmid (20 mg) were introduced into 25-day-old first or second castor true leaf cells using a Helios Gene Gun (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s instructions. During the bombardment,

(9) The temporal pattern of synaptic activation determines the longevity of structural plasticity at dendritic spinesiScienceJune 16, 2023Ali Özgür Argunsah, Inbal IsraelySigma unless otherwise indicated. Biolistic gene transfection Slice cultures were transfected using a Helios gene gun (Bio-Rad) after 4–5 days in vitro (DIV). Gold beads (10 mg, 1.6 μm diameter, Bio-Rad) were coated with

(10) Assessment of Lab4P Probiotic Effects on Cognition in 3xTg-AD Alzheimer’s Disease Model Mice and the SH-SY5Y Neuronal Cell LineInternational Journal of Molecular SciencesFebruary 28, 2023Thomas S. Webberley, Ryan J. Bevan, Joshua Kerry-Smith, Jordanna Dally, Daryn R. Michael, Sophie Thomas, Meg Rees, James E. Morgan, Julian R. Marchesi, Mark A. Good, et al.‘bullets’ for ballistic DiOlistic labelling onto fresh hippocampal slices (200 μm thick) at 100 psi using a Helios Gene Gun (Bio-Rad, USA) through a 3.0 µm pore size cell culture insert. Dye labelling was checked under a fluorescence microscope

(11) Concerted roles of LRRTM1 and SynCAM 1 in organizing prefrontal cortex synapses and cognitive functionsNature communicationsJanuary 28, 2023de Arce KP, Ribic A, Chowdhury D, Watters K, Thompson GJ, Sanganahalli BG, Lippard ETC, Rohlmann A, Strittmatter SM, Missler M, et al.1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; Invitrogen) and delivered using a Helios Gene Gun (BioRad, Hercules, CA). After incubation for 20 h at 4 °C in PBS, sections were fixed for 4–6 h and

(12) Chloroplast redox state changes mark cell‐to‐cell signaling in the hypersensitive responseNew PhytologistJanuary 1, 2023Tjaša Lukan, Anže Županič, Tjaša Mahkovec Povalej, Jacob O. Brunkard, Mirjam Kmetič, Mojca Juteršek, Špela Baebler, Kristina GrudenPVY‐N605(123)‐GFP was coated onto gold microcarriers and used for Nicotiana clevelandii bombardment with a Helios® gene gun (Bio‐Rad) according to Stare et al. (2020). Detailed PCR reaction conditions and cloning steps are given

(13) Brain-derived neurotrophic factor released from blood platelets prevents dendritic atrophy of lesioned adult central nervous system neuronsBrain communicationsJanuary 1, 2023Want A, Nan X, Kokkali E, Barde YA, Morgan JE.DiI-/DiO-coated tungsten particles were fired at the retinal wholemounts, ganglion cell side up, using a Helios gene gun (Bio-rad), 5 cm from the retinal surface, fired at 110 psi (helium) through a 3 µm polyethylene terephthalate

(14) Development of 89Zr-anti-CD103 PET imaging for non-invasive assessment of cancer reactive T cell infiltrationJournal for ImmunoTherapy of CancerDecember 5, 2022Arjan Kol, Xiaoyu Fan, Marta A. Wazynska, Sander M.J. van Duijnhoven, Danique Giesen, Annechien Plat, Hans Van Eenennaam, Philip H. Elsinga, Hans W. Nijman, Marco de Bruynfrom GeneArt/ThermoFisher (Regensburg, Germany). Mice were immunized by gene gun immunization using a Helios Gene gun (BioRad, Hercules, California, USA) and DNA coated gold bullets (BioRad) following manufacturer’s instructions

(15) An interactive time series image analysis software for dendritic spinesScientific ReportsDecember 1, 2022Ali Özgür Argunşah, Ertunç Erdil, Muhammad Usman Ghani, Yazmín Ramiro-Cortés, Anna F. Hobbiss, Theofanis Karayannis, Müjdat Çetin, Inbal Israely, Devrim Ünay\usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document}μg) or AFP using a Biorad Helios gene gun after 6 or 7 days in-vitro (DIV). Imaging experiments were performed 2 to 5 days post-transfection.

(16) Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivoNature MethodsNovember 1, 2022Crystian I. Massengill, Landon Bayless-Edwards, Cesar C. Ceballos, Elizabeth R. Cebul, James Cahill, Arpita Bharadwaj, Evan Wilson, Maozhen Qin, Matthew R. Whorton, Isabelle Baconguis, et al.~60% of the media. Transfection of cultured slices was accomplished by the biolistic method (Bio-Rad Helios gene gun) 10–20 days in vitro (DIV) using 1.6 μm gold particles (Bio-Rad, #165-2262; ~1 μg DNA/mg gold) coated

(17) Long-term potentiation reconstituted with an artificial TARP/PSD-95 complexCell reportsOctober 11, 2022Ravi AS, Zeng M, Chen X, Sandoval G, Diaz-Alonso J, Zhang M, Nicoll RA.assays,Critical commercial assays,Critical commercial assays ,, Helios Gene Gun Kit,Bio-Rad,Cat# 1652411 Helios Cartridge Kit,Bio-Rad,Cat# 1652440 ,, Experimental models: Cell lines,Experimental models:

(18) RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmissionScience advancesSeptember 30, 2022Ma P, Wan LP, Li Y, He CH, Song NN, Zhao S, Wang H, Ding YQ, Mao B, Sheng N.from P6 to P8 rats as previously described (52). Biolistic transfections were accomplished on DIV2 by Helios Gene Gun (Bio-Rad) with 1-μm DNA-coated gold particles. Slices were cultured at 34°C with medium, including 49%

(19) Cytosine Methylation in Genomic DNA and Characterization of DNA Methylases and Demethylases and Their Expression Profiles in Viroid-Infected Hop Plants (Humulus lupulus Var. ‘Celeia’)CellsAugust 19, 2022Andrej Sečnik, Nataša Štajner, Sebastjan Radišek, Urban Kunej, Mitja Križman, Jernej Jakšewith 360 ng of dimeric viroid constructs (GenBank X07397, GenBank KM211546, GenBank X07405) using the Helios GeneGun (Bio-Rad Laboratories, Inc., Hercules, CA, USA) [53,73]. Plants were placed in polyethylene bags to

(20) Disrupted Decision-Making: EcoHIV Inoculation in Cocaine Dependent RatsInternational Journal of Molecular SciencesAugust 13, 2022Kristen A. McLaurin, Hailong Li, Charles F. Mactutus, Steven B. Harrod, Rosemarie M. Booze(0.5 L per minute). The ballistic cartridges were cut into 13 mm lengths and stored in the dark. The Helios gene gun (Bio-Rad), which had a piece of filter paper between the two mesh screens, was loaded with the prepared

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